At present, learning modern Cell Biology relies on studying the images of cells and organelles in textbooks. All these images are taken from cells or tissues that have been chemically fixed (i.e. killed). Such chemical fixation can generate artifacts and only static images can be captured. Thus, such approach may not reflect the real cellular, physiological and dynamic responses in living cells or organisms. The recent development of green fluorescent protein (GFP) technology and advancement of high-resolution live-cell imaging systems have allowed real-time documentation of cellular dynamics and responses in transgenic organisms such as plants and animals. These dynamic studies of proteins, transport vesicles and organelles in living cells or tissues will further broaden our knowledge and understandings in cell biology. All these can be achieved by developing a multimedia self-learning kit of live cell imaging for Cell Biology learning in large classroom teaching.