There are still many unknown systemic factors which contribute to the pathogenesis of osteoporosis and its treatment. We sought to examine and compare the bone proteome of ovariectomized (OVX)/tail-suspension (TS) rats with normal rats. We found that Spp24 and fetuin-A appeared to be the most significant variations of proteins in osteopenic conditions induced by ovariectomy and tail-suspension. Our previous study demonstrated that tea can protect the bone. This further study confirmed tea polyphenol epigallocatechin (EGC) was a good bone protective agent thru downregulating the expression of Spp24.
Ganoderma sinense (GS) is a Chinese unique medicinal fungus has being used in the traditional Chinese medicine for a long history. A water-soluble protein-bound glucan GSP-2 was isolated and found to be immunomodulatory. The present study aimed to investigate how GSP-2 triggered the immunologic response and relevant downstream signaling pathway. Our results demonstrated that GSP-2 activated TLR4 overexpression cell line. GSP-2 could upregulate the TLR4 protein expression, at the same time the PCR array results suggested GSP-2 can stimulate the TLR4 downstream signaling pathway, and then induce the cytokine, as TNFα, IL1β and IL6, production in RAW264.7 macrophage.
In collaboration with China Astronaut Research and Training Centre (CARTC), we investigated the effectiveness of TCM formula on muscoluskeletal system, immune function and cardio pulmonary function of astronauts. In parallel to clinical bed-rest investigation, we employed a hindlimb unloaded (tail-suspension) rat model to mimic the weightlessness condition on hindlimb. We found that taikong yangxin prescription (TKYXP) protect the bone under weightless influence from gradual structural deterioration. TKYXP also could boost up the immune system and improve the cardiac functions.